Precipitation and inhibition of carboxypeptidase by specific antisera.
نویسندگان
چکیده
Quantitative immunochemical techniques have been employed in the study of enzyme systems as a method of assessing the homogeneity of the enzyme protein. In addition, when the antibody acts as an enzyme inhibitor, useful information can frequently be obtained. Such studies have recently been reviewed by Marrack (1). With crystalline carboxypeptidase as an antigen, specific antibodies were obtained. The enzyme was found to fulfil most of the criteria for a homogeneous substance in quantitatilre precipitin tests and with the agar diffusion technique. In homogeneous solution, the ant,ibody interacted slowly with carboxypeptidase to produce a non-competitive inhibition of the catalytic activity. Certain properties of these syst)ems were investigated.
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 199 2 شماره
صفحات -
تاریخ انتشار 1952